This proposal written by me to apply in following project of Dr . Sidra ijaz university of Agriculture Faisalabad.
Title of project:
Resistance gene analogues based molecular
identification for dieback disease resistance in
Shisham (Dalbergiasissoo).
Objectives of project
·
Raise the importance of shisham tree (Dalbergiasissoo).
·
To reveal the cause of its syndrome (dieback
disease).
·
Give the information about its major
pathogens.
·
Molecular identification of its genes
which are having resistance against dieback disease.
·
Uses of all possible molecular and
biotechnological techniques to eliminate this disease.
Concept of project:
Shisham (Dalbergiasissoo)
is afrugallyvitalflexible deciduous tree species of family Papilionaceae (Singh
et al., 2011). The wood of shisham wood is very stiff and is best fit for
furniture (Hossain& Martin, 2013). In calculation, diverse parts of this
plant have medicinal status (Poojaet al., 2010; Asif& Kumar, 2011).
Unfortunatelyit is affected by dieback disease.Dieback
is disease in which marginal parts of trees and woody plants are killed due to
attack of pathogens, parasites and a weather condition like acid rain . But in
case of shisham,in the
initial phase, the tree which is affected is categorized by drooping leaves and
branches, due to loss of turgor.The Leaflets become yellow, desiccate and
ultimately drop off execution the branches naked. The whole tree converts thin
in difference to the connecting thick green tree. The tree which is affected by
this disease is resulted into death within six month .After the crown shows the
symptoms of wilt. Fusariumsolani is the causal agent (Bakhshi, 1954;Manandhar&Shrestha,
2000; Bajwaet al., 2003). The causal agent is limited to roots. The fungal hyphae and gel like constituents
wad the containers resultant in wilt Symptoms (Bakhsai& Singh, 1959).
. Others include pentatricopeptide repeats and apoplastic peroxidases.
Several plant-pathogen connections
are determined by the existence of resistance (R) genes/alleles
That enables plants to identify
pathogens and trigger inducible resistances. NDR1 and three alleles of rp1,
rp1-D21, rp1-MD19 and rp-NC3, have been shown to confer
non-specific resistance response to rust.
References:
·
J.Arshad; Bajwa.R; Shah.M.B.M. Dieback Resistance Potential in Different Varieties
of Shisham (DalbergiasissooRoxb.Mycopath, 2003, 1(2):
105-110.
·
Manoj.K.S; Li .P; Irene.L
;Wang.X; et al.Disease Resistance Gene Analogs (RGAs) in Plants.Int. J. Mol. Sci. 2015, 16,
19248-19290; doi:10.3390/ijms160819248.
·
